Vaccinia virus DNA replication: two hundred base pairs of telomeric sequence confer optimal replication efficiency on minichromosome templates.

Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication

The genetic information encoded in genomes must be faithfully replicated and transmitted to daughter cells. The recent discovery of consecutive DNA conversions by TET family proteins of 5-methylcytosine into 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) suggests these modified cytosines act as DNA lesions, which could threaten genome integrity. Here, we have shown tha...

Low-resolution structure of vaccinia virus DNA replication machinery.

Smallpox caused by the poxvirus variola virus is a highly lethal disease that marked human history and was eradicated in 1979 thanks to a worldwide mass vaccination campaign. This virus remains a significant threat for public health due to its potential use as a bioterrorism agent and requires further development of antiviral drugs. The viral genome replication machinery appears to be an ideal ...

Studies on the replication of African Horse Sickness Virus in two different cell line cultures

Attenuated and replication competent Vaccinia Virus strains

16 Replication competent poxvirus vectors with an attenuation phenotype and with high 17 immunogenic capacity of the foreign expressed antigen are being pursuit as novel 18 vaccine vectors against different pathogens. In this investigation we have examined the 19 replication and immunogenic characteristics of two vaccinia virus (VACV) mutants 20 M65 and M101. These mutants were generated after ...

Complete in vitro reconstitution of adeno-associated virus DNA replication requires the minichromosome maintenance complex proteins.

Adeno-associated virus (AAV) replicates its DNA exclusively by a leading-strand DNA replication mechanism and requires coinfection with a helper virus, such as adenovirus, to achieve a productive infection. In previous work, we described an in vitro AAV replication assay that required the AAV terminal repeats (the origins for DNA replication), the AAV Rep protein (the origin binding protein), a...